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KMID : 0613820020120050570
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Journal of Life Science
2002 Volume.12 No. 5 p.570 ~ p.576
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Amylase Production from Haloarcular sp. EH-1
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Jung Myung-Ju
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Abstract
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The extremely halophilic archaebacterium Haloarcular sp. EH-1 was isolated from solar salts. Amylae production from Haloarcular sp. EH-1 have been studied. The results obtained were as follows. The optimal medium composition for the production of amylase from Haloarcular sp. EH-1 were soluble starch 1.5%, yeast extract 1.0%, MgSO4ㆍ7H2O 2.0%, KCl 0.1%, NaCl 25% (pH 7.5). The incubation temperature, aeration rate and agitation speed were 40℃, 100 ㎖ medium/ 500 ㎖ shaking flask, and 110 rpm. The cell growth and enzymatic activity was highest at 9 days of incubation. So amylase production appeared to be a growth-related phenomenon.
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KEYWORD
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extremely halophilic bacteria, Haloarcular sp., archaebacteria, amylase
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